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KMID : 0363320090300020288
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Journal of Korean Oriental Internal Medicine
2009 Volume.30 No. 2 p.288 ~ p.297
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Anti-inflammatory Effects of Smilacis Glabrae Rhizoma in Raw 264.7 Cells
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Oh Sung-Won
Kim Byoung-Woo
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Abstract
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Objective: Inflammatory cytokines have a close relationship to insulin dependent diabetes mellitus (IDDM). The inhibitory effect of Smilacis Glabrae Rhizoma (SGR) were examined on production of nitric oxide (NO), prostaglandin , synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor- (TNF-) and NF-B activation in Raw 264.7 cells.
Methods: Raw 264.7 cells were pretreated with SGR(20, 50, 100 /ml), and then cultured with lipopolysaccharides (LPS). Cell viability was measured by MTT assay; inhibition of NO, , and TNF- production were measured by Griess reagent and enzyme-linked immunosorbent assay(ELISA). Induction of COX-2 and iNOS were determined by western blotting analysis. Inhibition of NF-B was measured by immunofluorescence assay (IFA).
Results: SGR inactivated NF-B, and inhibited the production of NO, iNOS, and . Inhibition of COX-2 and TNF- could not be confirmed.
Conclusions: From the above result. SGR was found to have an anti-inflammatory effect of inhibition of NO, iNOS, and production via inhibition of NF-B.
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KEYWORD
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Anti-inflammatory, Smilacis Glabrae Rhizoma, nitric oxide, prostaglandin , iNOS, cyclooxygenase-2
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